# GABA caged for studying $GABA_{A}$ receptor in cerebellar granule cells $in vitro$.

Gatta E., Bazzurro V., Cozzolino M., Bianchini P., Cupello A., Diaspro A., Robello M.

Comunicazione
V - Biofisica e fisica medica
Aula 32C-2 - Martedì 18 h 09:00 - 13:00
GABA caged is a photoactivatable molecule whose activity is controlled by a light pulse. Uncaging technology and fluorescence microscopy coupled to patch clamp technique provide an approach to detect a selected biological target in a temporally and spatially confined way. The combination of electrophysiological and fluorescence recordings allows monitoring biochemical and electrical signals simultaneously. Our goal is to study the release of GABA caged topically applied in a specific region for mapping the localization and distribution of $GABA_{A}$ receptors in neurons $in vitro$. Localization precision can be improved by using advanced fluorescent optical methods as in super resolved and non-linear fluorescence microscopy.