Kinetic and dynamic characterization of the interaction between Staphylococcus aureus IsdB and human hemoglobin.
Moscetti I., Bettati S., Bizzarri A., Campanini B., Cannistraro S., De Bei O., Gianquinto E., Luque J.F., Marchetti M., Spyrakis F., underline Ronda L.
V - Biofisica e fisica medica
GSSI Ex ISEF - Aula B - Martedì 24 h 17:30 - 19:30
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$Staphylococcus aureus$ exploits different mechanisms for iron supply, but the preferred one is the acquisition of organic iron through the expression of hemoglobin (Hb) receptors. One of these, IsdB, is a recognized virulence factor and its interaction with Hb represents a target for the design of antibacterial molecules. However, many structural and mechanistic details of complex formation are still elusive. By combining absorption and fluorescence spectroscopy, surface plasmon resonance and computational methods, we investigated i) the exact complex stoichiometry, ii) the microscopic kinetic rates of complex formation and heme extraction, iii) IsdB selectivity for Hb alpha and beta subunits, and iv) the structural/dynamic effect played by the hemophores on Hb.