Dynamic coupling at the protein-lipid interface in membranes.
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V - Biofisica e fisica medica
Aula 32C-2 - Lunedì 17 h 16:00 - 19:00
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The function of integral membrane proteins depends on the surrounding lipids. The interfacial lipids mediate the dynamical properties in the nano- to microsecond timescale between the protein and the membrane environment. By using continuous wave and pulsed EPR spectroscopy small angle fast librational motion and larger scale motion are selectively detected on the protein, the membrane and the extracted lipid component of a membrane-bound Na,K-ATPase enzyme. The onset of the protein librational motion is correlated with the interconversion between conformational substates that ultimately lie on the pathways in the enzymatic cycle. Similarities with solvent-mediated dynamics of soluble proteins are discussed.