Single-molecole analysis of DNA nanomechanics.
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V - Biofisica e fisica medica
Aula 32C-2 - Giovedì 20 h 16:30 - 19:00
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During its physiological activity, the DNA molecule, typically found in B-structure, explores different forms, whose transitions between each other are induced by the enzymatic activity that often exerts nanomechanical stress on the molecule. In my laboratories, by means of a Magnetic-Tweezers (MT) apparatus, we are studying the DNA structural transitions that occur at different, positive or negative supercoiling. The unique possibility of the setup to apply controlled torques and forces to single molecules allows probing the DNA in a controlled way. Furthermore, by calculating the mechanical parameters and by measuring directly the energy to induce the transition, it is possible to quantify the energy gap between the different DNA phases.