Relazione su invito
Second-harmonic generation study of collagen irradiated scaffolds.
Croci S., Manghi M., Bruni L., Lamparelli E.P., Della Porta G., Bellini D.
3D culture models are expected to introduce substantial advantages over cell monolayers because they open up the possibility of shifting radiobiological investigations from studies of cellular targets to the investigation of more complex cellular responses involving the network of signals arising from cell-cell and cell-matrix interactions. The first unavoidable step is to understand the radiation effects on the 3D scaffolds which could be used to culture cells. We start to investigate as possible 3D substrates the BIOPADTM and HyCo collagen pads used in bioengineering. These "sponges" are formed of native equine collagen that retains its triple helix structure allowing visualization of second harmonic generated. Results on scaffolds irradiated with protons and electrons will be presented. The irradiations are conducted in conventional and in ultra high dose rate, above 100 Gy/s, (FLASH) modalities.