Morpho-mechanics of collagen superstructures revealed by Brillouin-Raman microspectroscopy.

Brillouin and Raman spectroscopy are established optical techniques for the nondestructive contactless and label-free readout of mechanical, chemical, and structural properties of condensed matter. These techniques, coupled with confocal microscopy, have recently gained renewed interest in 2D and 3D mapping of viscoelastic properties and molecular composition of biological matter. Here we report some recent advances of a multimodal scanning technique, the Brillouin-Raman micro-spectroscopy, for biomedical applications with a focus on the morpho-mechanical analysis of cornea tissues.